DNP Methods for Cardiac Metabolic Imaging with Hyperpolarized [1-13C]pyruvate Large Dose Injection in Pigs

In this study we set up and optimized a dynamic nuclear polarization (DNP) procedure for the hyperpolarization of a large dose suitable for cardiac metabolic imaging in vivo in swine models. The dose range proposed in this study allowed chemical shift imaging of cardiac metabolism with hyperpolarized [1-C]pyruvate in pigs. We investigated the typical pattern of distribution of [1-C]pyruvate and its downstream metabolites: we analysed the variation of the maximum value of the normalized [1-C]pyruvate signal and the global pyruvate signal in left ventricle (LV). The study reports data obtained with a large dose increase compared to small A. Flori V. Lionetti F. A. Recchia Istituto di Scienze della Vita, Scuola Superiore Sant’Anna, Pisa, Italy F. Frijia V. Positano L. Landini M. F. Santarelli M. Lombardi L. Menichetti Fondazione G.Monasterio CNR-Regione Toscana, Pisa, Italy J. H. Ardenkjaer-Larsen GE Healthcare, Hillerod, Denmark G. Giovannetti M. F. Santarelli L. Menichetti (&) CNR Institute of Clinical Physiology, Pisa, Italy e-mail: [email protected] R. F. Schulte F. Wiesinger GE Global Research, Munich, Germany F. A. Recchia Department of Physiology, New York Medical College, Valhalla, New York, NY, USA L. Landini Department of Electronic Engineering, University of Pisa, Pisa, Italy J. H. Ardenkjaer-Larsen Department of Electrical Engineering, Technical University of Denmark, Kongens Lyngby, Denmark 123 Appl Magn Reson (2012) 43:299–310 DOI 10.1007/s00723-012-0350-9 Applied